プライマ−リスト(PCR用)
T3 や T7 などよく使われるプライマーを大量合成し、小分けしたものです。濃度100ピコモル/マイクロリットルで、100マイクロリットルずつ分注してあります(Total 10 nmol)。PCR用です。sequence反応に使用出来るかどうかのチェックは行っていません。1本700円です。
支払方法については、原則として、機械を利用する場合の利用者負担金と同様に年1回集計し、移算手続きを行うことにします。
No. | Name | Size | Sequence | Note |
1 | T3 | 21 mer | 5'-att aac cct cac taa agg gaa-3' | pBluescript II |
2 | T7 | 20 mer | 5'-taa tac gac tca cta tag gg-3' | pBluescript II |
3 | M13-F | 20 mer | 5'-gtt gta aaa cga cgg cca gt-3' | M13 or pUC |
4 | M13-R | 21 mer | 5'-agg aaa cag cta tga cca tga-3' | M13 or pUC |
5 | λ11-F | 24 mer | 5'-ggt ggc gac gac tcc tgg agc ccg-3' | λgt11 |
6 | λ11-R | 24 mer | 5'-ttg aca cca gac caa ctg gta atg-3' | λgt11 |
7 | λ10-F | 20 mer | 5'-gct ggg tag tcc cca cct tt-3' | λgt10 |
8 | λ10-R | 24 mer | 5'-ctt atg agt att tct tcc agg gta-3' | λgt10 |
9 | SP6 | 24 mer | 5'-cat acg att tag gtg aca cta tag-3' | SP6 promoter |
10 | Z-1 | 19 mer | 5'-gcg tta ccc aac tta atc g-3' | lacZ gene |
11 | Z-2 | 20 mer | 5'-tgt gag cga gta aca acc cg-3' | lacZ gene |
12 | CAT-2 | 18 mer | 5'-act ggt gaa act cac cca-3' | chloramphenicol acetyltransferase (CAT) gene |
13 | CAT-3 | 21 mer | 5'-cag tca gtt gct caa tgt acc-3' | chloramphenicol acetyltransferase (CAT) gene |
14 | neo-F | 20 mer | 5'-aga ggc tat tcg gct atg ac-3' | neomycin resistance gene [GenBank Accession:U43611] |
15 | neo-R | 20 mer | 5'-cac cat gat att cgg caa gc-3' | neomycin resistance gene [GenBank Accession:U43611] |
16 | mG3PDH-1 | 19 mer | 5'-gga aag ctg tgg cgt gat g-3' | mouse G3PDH gene [GenBank Accession:M32599] |
17 | mG3PDH-2 | 20 mer | 5'-ctg ttg ctg tag ccg tat tc-3' | mouse G3PDH gene [GenBank Accession:M32599] |
18 | Cre-1 | 20 mer | 5'-aca tgt tca ggg atc gcc ag-3' | Cre gene |
19 | Cre-2 | 21 mer | 5'-taa cca gtg aaa cag cat tgc-3' | Cre gene |
20 | Cre-3 | 20 mer | 5'-aat gct tct gtc cgt ttg cc-3' | Cre gene |
21 | Cre-4 | 20 mer | 5'-cta cac cag aga cgg aaa tc-3' | Cre gene |
22 | ori-1 | 20 mer | 5'-aga ggc ggt ttg cgt att gg-3' | pUC origin |
23 | ori-2 | 20 mer | 5'-cag tgg cga taa gtc gtg tc-3' | pUC origin |
24 | BG-1 | 21 mer | 5'-atc cag gtt aca agg cag ctc-3' | mouse beta-globin gene (intron) |
25 | BG-2 | 21 mer | 5'-tta ccc tca ctt cca tcc ctc-3' | mouse beta-globin gene (intron) |
26 | BG-3 | 21 mer | 5'-aga aca gag tga gag gga gca-3' | mouse beta-globin gene (intron) |
27 | loxP-B | 22 mer | 5'-gat ccg gaa ccc tta ata taa c-3' | loxP site |
28 | loxP-P | 20 mer | 5'-agg tcg agg gac cta ata ac-3' | loxP site |
29 | lox66-B | 21 mer | 5'-atc cgg aac cct taa tta ccg-3' | mutant lox site |
30 | lox71-P | 19 mer | 5'-ggt cga ggg acc tat acc g-3' | mutant lox site |
31 | En2-SA | 20 mer | 5'-atg aca acc agg tcc cag gt-3' | En2 gene splice accepter |
32 | 34W-1 | 34 mer | 5'-ctg ttt gct acc taa aga agg cct ttt ttc tgg t-3' | 34 bp size marker |
33 | 34W-2 | 34 mer | 5'-acc aga aaa aag gcc ttc ttt agg tag caa aca g-3' | 34 bp size marker |
34 | HBx-1 | 20 mer | 5'-tct acc gtc ccc ttc ttc at-3' | HBV HBx gene |
35 | HBx-2 | 21 mer | 5'-caa ttt atg cct aca gcc tcc-3' | HBV HBx gene |
36 | Ori-3 | 21 mer | 5'-cac aga atc agg gga taa cgc-3' | pUC origin |
37 | EL-9 | 20 mer | 5'-cct aga cgt tgt aag aag gc-3' | mouse E-selectin |
38 | EL-11 | 20 mer | 5'-gat tgg aca ctc aat gga tc-3' | mouse E-selectin |
39 | bsr-1 | 21 mer | 5'-ctt ctc tgt cgc tac ttc tac-3' | blasticidin S-resistance gene (bsr) |
40 | bsr-2 | 20 mer | 5'-gca gaa atc gga gga aga ag-3' | blasticidin S-resistance gene (bsr) |
41 | bsr-3 | 20 mer | 5'-caa ctc cct aca cat acc ac-3' | blasticidin S-resistance gene (bsr) |
42 | AC-1 | 19 mer | 5'-tcg gct tct ggc gtg tga c-3' | AC promoter |
43 | AG-1 | 20 mer | 5'-ggt tgt tgt gct gtc tca tc-3' | rabbit beta-globin partial genomic DNA (intron/polyA signal) |
44 | AG-2 | 20 mer | 5'-ctg cta acc atg ttc atg cc-3' | rabbit beta-globin partial genomic DNA (intron/polyA signal) |
45 | GFP-1 | 20 mer | 5'-tac ggc aag ctg acc ctg aa-3' | GFP gene |
46 | GFP-2 | 20 mer | 5'-tgt gat cgc gct tct cgt tg-3' | GFP gene |
47 | AAT-7 | 18 mer | 5'-gtg tct aag gct gac tgg-3' | human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257] |
48 | AAT-21 | 20 mer | 5'-cct ggt ctg aat gtg tgt gc-3' | human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257] |
49 | AAT-31 | 18 mer | 5'-tgt ccc agg tca gtg gtg-3' | human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257] |
50 | PAC-1 | 20 mer | 5'-tcc atg acc gag tac aag cc-3' | puromycin N-acetyltransferase (pac) gene |
51 | PAC-2 | 20 mer | 5'-ggc ctt cca tct gtt gct gc-3' | puromycin N-acetyltransferase (pac) gene |
52 | bsd-1 | 19 mer | 5'-ggt ctc cca tgc ctt tgt c-3' | blasticidin S-resistance gene (bsr) |
53 | bsd-2 | 19 mer | 5'-cac ata acc aga ggg cag c-3' | blasticidin S-resistance gene (bsr) |
54 | AG-3 | 18 mer | 5'-gat cct gag aac ttc agg-3' | rabbit beta-globin partial genomic DNA (intron/polyA signal) |
55 | AG-4 | 19 mer | 5'-acc acc ttc tga tag gca g-3' | rabbit beta-globin partial genomic DNA (intron/polyA signal) |
56 | AG-5 | 19 mer | 5'-tag gca gcc tgc acc tga g-3' | rabbit beta-globin partial genomic DNA (intron/polyA signal) |
57 | AG-6 | 20 mer | 5'-cag aag tca gat gct caa gg-3' | rabbit beta-globin partial genomic DNA (intron/polyA signal) |
58 | RGpA-1 | 20 mer | 5'-tca tag ctg tcc ctc ttc tc-3' | rabbit beta-globin partial genomic DNA (intron/polyA signal) |
59 | RGpA-2 | 21 mer | 5'-ctg ctg tcc att cct tat tcc-3' | rabbit beta-globin partial genomic DNA (intron/polyA signal) |
60 | AG3AS | 19 mer | 5'-cct gaa gtt ctc agg atc c-3' | rabbit beta-globin partial genomic DNA (intron/polyA signal) |
61 | pSP72-1 | 19 mer | 5'-ggg ctg gct tta cta tgc g-3' | pSP72 |
62 | pSP72-2 | 20mer | 5'-ata gtt aag cca gcc ccg ac-3' | pSP72 |
63 | pSP72-3 | 20 mer | 5'-ata agg gcg aca cgg aaa tg-3' | pSP72 |
64 | pSP72-4 | 20mer | 5'-agt aag ttg gcc gca gtg tt-3' | pSP72 |
65 | pSP72-5 | 21mer | 5'-tca cgt taa ggg att ttg gtc-3' | pSP72 |
66 | pSP72-6 | 20 mer | 5'-tgt agg tat ctc agt tcg gt-3' | pSP72 |
67 | AAT-8 | 20 mer | 5'-acc tgg gac agt cta gaa ct-3' | human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257] |
68 | AAT-9 | 20 mer | 5'-ttg ccc ctt ctg tca ttc ac-3' | human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257] |
69 | pUpA | 20mer | 5'-tat tcc ttt gcc ctc gga cg-3' | mouse PGK gene polyadenylation signal |
70 | pUpA-S | 24mer | 5'-gca gaa att gat gat cta tta aac-3' | mouse PGK gene polyadenylation signal |
71 | SA-1 | 24 mer | 5'-tcc tct ttg tta ggg ttc ttc ttc-3' | mouse Engraled-2 (En2) gene splice acceptor |
72 | SA-2 | 19 mer | 5'-gcc ctt tct cct cca tga c-3' | mouse Engraled-2 (En2) gene splice acceptor |
73 | SA-3 | 20 mer | 5'-gca aac gag gcc aga aag at-3' | mouse Engraled-2 (En2) gene splice acceptor |
75 | SA-5 | 21mer | 5'-ggt cac ttt atg ttc ttg ccc-3' | mouse Engrailed-2 (En2) gene splice acceptor |
76 | SA-6 | 20mer | 5'-agg tct caa gtt tta gcc gg-3' | mouse Engrailed-2 (En2) gene splice acceptor |
77 | SA-7 | 20mer | 5'-atg tcc tct ggt gct caa ag-3' | mouse Engrailed-2 (En2) gene splice acceptor |
78 | SA-8 | 20 mer | 5'-ata gga gtt tgc gga ggt tg-3' | mouse Engrailed-2 (En2) gene splice acceptor |
79 | SA-9 | 28 mer | 5'-ata gtc gac ttc ttc ttt ggt ttt cgg g-3' | mouse Engrailed-2 (En2) gene splice acceptor |
80 | SA-10 | 19 mer | 5'-agc agt gaa ggc tgt gcg a-3' | mouse Engrailed-2 (En2) gene splice acceptor |
81 | SA-11 | 20 mer | 5'-ccc gaa aac caa aga aga ag-3' | mouse Engrailed-2 (En2) gene splice acceptor |
82 | LZUS-3 | 19 mer | 5'-cgc atc gta acc gtg cat c-3' | lacZ gene |
83 | STOP-1 | 29 mer | 5'-taa ctg agt aga cta gtc tag aat tcg gc-3' | universal terminator |
84 | STOP-2 | 33 mer | 5'-tcg agc cga att cta gac tag tct act cag tta-3' | universal terminator |
85 | polyT-Xho | 24 mer | 5'-ccc ctc gag ttt ttt ttt ttt ttt-3' | polyT(15) + XhoI site |
86 | polyT20-Xho | 29 mer | 5'-ccc ctc gag ttt ttt ttt ttt ttt ttt tt-3' | polyT(20) + XhoI site |
87 | VC-11 | 20mer | 5'-caa cga tct ctg tac atc cc-3' | vascular cell adhesion molecule (VCAM) |
88 | VC-12 | 20mer | 5'-aga ggc tgt aca ctc tgc ct-3' | vascular cell adhesion molecule (VCAM) |
89 | VC-13 | 21mer | 5'-ccc ttg aac aga tca atc tcc-3' | vascular cell adhesion molecule (VCAM) |
90 | VC-15 | 20mer | 5'-gca gag act tga aat gcc tg-3' | vascular cell adhesion molecule (VCAM) |
91 | Amp-S | 20mer | 5'-tac agg cat cgt ggt gtc ac-3' | ampicillin resistance gene |
92 | Amp-AS | 20mer | 5'-aaa tgt gcg cgg aac ccc ta-3' | ampicillin resistance gene |
93 | Primer3 | 24mer | 5'-ccc gtg gtg gtg ttc gac ttt gcc-3' | HSV |
94 | Primer4 | 24mer | 5'-gca caa aga tgg agt ccg tgt ccc-3' | HSV |
95 | 154-1 | 23mer | 5'-ttc ttc gtc aag gct cac gtg cg-3' | HSV |
96 | 154-2 | 25mer | 5'-ccg agt tac aca cga cct tga tgg c-3' | HSV |
97 | 297-1 | 26mer | 5'-aag gtc gtg tgt aac tcg gtg tac gg-3' | HSV |
98 | 297-2 | 23mer | 5'-gat gat gcg cat gga ata ggg cc-3' | HSV |
99 | hyg-pA | 20mer | 5'-tgc ctt cct tga ccc tgg aa-3' | pIRES1hyg |
100 | FRT-S | 20mer | 5'-gct tca aaa gcg ctc tga ag-3' | FRT |
101 | AUAP | 20mer | 5'-ggc cac gcg tcg act agt ac-3' | abridged universal amplification primer for 5'-RACE |
102 | Flp-2 | 20mer | 5'-tcg gaa gaa gca gat aag gg-3' | Flp gene |
103 | Flp-5 | 20mer | 5'-atc cta ccc ctt gcg cta aa-3' | Flp gene |
104 | Z-5 | 20mer | 5'-acg gcg gat tga ccg taa tg-3' | lacZ gene |
105 | PGKpA | 22mer | 5'-aac ttccat ttt agt gga cat c-3' | pU-21T pA2 |
106 | SA-12 | 20mer | 5'-gga aac aaa ctt ggc ctc ac-3' | mouse Engraled-2 (En2) gene splice acceptor |
107 | SA-13 | 20mer | 5'-cct gtt ggt ctg aaa ctc ag-3' | mouse Engraled-2 (En2) gene splice acceptor |
108 | Z-6 | 20mer | 5'-gcg att aag ttg ggt aac gc-3' | lacZ gene |
109 | GFP-3 | 20mer | 5'-acg aga agc gcg atc aca tg-3' | GFP gene |
110 | Z-3 | 19mer | 5'-ggc ctc ttc gct att acg c-3' | lacZ gene |
111 | Z-7 | 20mer | 5'-tct ggc ctt cct gta gcc ag-3' | lacZ gene |
112 | Z-8 | 21mer | 5'-gtt tta caa cgt cgt gac tgg-3' | lacZ gene |
113 | SV40pA-S | 21mer | 5'-gcc ata cca cat ttg tag agg-3' | pU-21T pA1 |
114 | DT-A-1 | 20mer | 5'-tac cac ggg act aaa cct gg-3' | DT-A gene |
115 | DT-A-2 | 20mer | 5'-cgc tta acg ctt tcg cct gt-3' | DT-A gene |
116 | DT-A-3 | 21mer | 5'-gtt gat gga gca agt cgg aac-3' | DT-A gene |
117 | DT-A-4 | 21mer | 5'-cct tgt gta cca gat ttt ggc-3' | DT-A gene |
118 | DT-A-5 | 20mer | gaa aac gtg gcc aag atg cg-3' | DT-A gene |
119 | PGKpro | Size | Sequence | Note |
120 | PGKpA-S | 22mer | 5'-aag atg tcc act aaa atg gaa g-3' | pU-21T pA2 |
121 | GFP-1AS | 20mer | 5'-ttc agg gtc agc ttg ccg ta-3' | GFP gene |
122 | SA14AS | 21mer | 5'-cac atc cat gct gag gat gag-3' | mouse Engraled-2 (En2) gene splice acceptor |
123 | SA-ter-Kp | 32mer | 5'-atg gta cct aag cag tga agg cta tgc gag gc-3' | mouse Engraled-2 (En2) gene splice acceptor |
124 | WZ-14 | 21mer | 5'-gct ctc tac agg tgg atc aag-3' | pU-21W |
125 | WZ-17 | 21mer | 5'-gca gtt caa tca gct gct ttc-3' | pU-21W |
126 | SA-125 | 20mer | ctc ctg gga act cta ctg cc-3' | mouse Engraled-2 (En2) gene splice acceptor |
127 | SA-125AS | 20mer | ggc agt aga gtt ccc agg ag | mouse Engraled-2 (En2) gene splice acceptor |
128 | pSP72-4R | 20mer | 5'-aac act gcg gcc aac tta ct-3' | pSP72 |
129 | LPBL | 27mer | 5'-gga tcc gga acc ctt aat ata act tcg-3' | mouse Engraled-2 (En2) gene splice acceptor |
130 | Sry-F | 20mer | 5'-tga ctg gga tgc agt agt tc-3' | mouse Sry gene |
131 | Sry-R | 20mer | 5'-tgt gct aga gag aaa ccc tg-3' | mouse Sry gene |
132 | pSP72-7 | 21mer | 5'-tca agg atc tta ccg ctg ttg-3' | pSP72 |
133 | pSP72-8 | 21mer | 5'-gtg aaa acc tct gac aca tgc-3' | pSP72 |
134 | 3RACE-AP | 30mer | 5'-ggc cac gcg tcg act agt act ttt ttt ttt-3' | 3'-RACE |
135 | SA12-AS | 20mer | 5'-gtg agg cca agt ttg ttt cc-3' | mouse Engraled-2 (En2) gene splice acceptor |
136 | SA14 | 21mer | 5'-ctc atc ctc agc atg gat gtg-3' | mouse Engraled-2 (En2) gene splice acceptor |
137 | SA8-AS | 20mer | 5'-caa cct ccg caa act cct at-3' | mouse Engraled-2 (En2) gene splice acceptor |
138 | iCre-1 | 20mer | 5'-agg cct tct ctg aac aca cc-3' | iCre |
139 | iCre-2 | 21mer | 5'-ttg gtc ctg cca atg tgg atc-3' | iCre |
140 | SA3AS | 20mer | 5'-atc ttt ctg gcc tcg ttt gc-3' | mouse Engraled-2 (En2) gene splice acceptor |
141 | SA3.5AS | 23mer | 5'-aag agt agg cta gcc agt caa gg-3' | mouse Engraled-2 (En2) gene splice acceptor |
142 | SA4.5AS | 25mer | 5'-ttc ctg tcc agg aga ggg acc tgg c-3' | mouse Engraled-2 (En2) gene splice acceptor |
143 | SA5AS | 21mer | 5'-ggg caa gaa cat aaa gtg acc-3' | mouse Engraled-2 (En2) gene splice acceptor |
144 | SA6AS | 22mer | 5'-ccg gct aaa act tga gac ctt c-3' | mouse Engraled-2 (En2) gene splice acceptor |
145 | SA7AS | 23mer | 5'-tct ttg agc acc aga gga cat cc-3' | mouse Engraled-2 (En2) gene splice acceptor |
No. | Name | Size | Sequence | Note |
[更新2014.10.27]