プライマ−リスト(PCR用)

 T3 や T7 などよく使われるプライマーを大量合成し、小分けしたものです。濃度100ピコモル/マイクロリットルで、100マイクロリットルずつ分注してあります(Total 10 nmol)。PCR用です。sequence反応に使用出来るかどうかのチェックは行っていません。1本700円です。
 支払方法については、原則として、機械を利用する場合の利用者負担金と同様に年1回集計し、移算手続きを行うことにします。

No.NameSizeSequenceNote
1T3 21 mer5'-att aac cct cac taa agg gaa-3'pBluescript II
2T7 20 mer5'-taa tac gac tca cta tag gg-3'pBluescript II
3M13-F20 mer5'-gtt gta aaa cga cgg cca gt-3'M13 or pUC
4M13-R21 mer5'-agg aaa cag cta tga cca tga-3'M13 or pUC
5λ11-F24 mer5'-ggt ggc gac gac tcc tgg agc ccg-3'λgt11
6λ11-R24 mer5'-ttg aca cca gac caa ctg gta atg-3'λgt11
7λ10-F20 mer5'-gct ggg tag tcc cca cct tt-3'λgt10
8λ10-R24 mer5'-ctt atg agt att tct tcc agg gta-3'λgt10
9SP624 mer5'-cat acg att tag gtg aca cta tag-3'SP6 promoter
10Z-119 mer5'-gcg tta ccc aac tta atc g-3'lacZ gene
11Z-220 mer5'-tgt gag cga gta aca acc cg-3'lacZ gene
12CAT-218 mer5'-act ggt gaa act cac cca-3'chloramphenicol acetyltransferase (CAT) gene
13CAT-321 mer5'-cag tca gtt gct caa tgt acc-3' chloramphenicol acetyltransferase (CAT) gene
14neo-F20 mer5'-aga ggc tat tcg gct atg ac-3' neomycin resistance gene [GenBank Accession:U43611]
15neo-R20 mer5'-cac cat gat att cgg caa gc-3' neomycin resistance gene [GenBank Accession:U43611]
16mG3PDH-119 mer5'-gga aag ctg tgg cgt gat g-3'mouse G3PDH gene [GenBank Accession:M32599]
17mG3PDH-2 20 mer5'-ctg ttg ctg tag ccg tat tc-3'mouse G3PDH gene [GenBank Accession:M32599]
18Cre-120 mer5'-aca tgt tca ggg atc gcc ag-3'Cre gene
19Cre-221 mer5'-taa cca gtg aaa cag cat tgc-3'Cre gene
20Cre-320 mer5'-aat gct tct gtc cgt ttg cc-3'Cre gene
21Cre-420 mer5'-cta cac cag aga cgg aaa tc-3'Cre gene
22ori-120 mer5'-aga ggc ggt ttg cgt att gg-3'pUC origin
23ori-220 mer5'-cag tgg cga taa gtc gtg tc-3'pUC origin
24BG-121 mer5'-atc cag gtt aca agg cag ctc-3'mouse beta-globin gene (intron)
25BG-221 mer5'-tta ccc tca ctt cca tcc ctc-3'mouse beta-globin gene (intron)
26BG-321 mer5'-aga aca gag tga gag gga gca-3'mouse beta-globin gene (intron)
27loxP-B22 mer5'-gat ccg gaa ccc tta ata taa c-3'loxP site
28loxP-P20 mer5'-agg tcg agg gac cta ata ac-3'loxP site
29lox66-B21 mer5'-atc cgg aac cct taa tta ccg-3'mutant lox site
30lox71-P19 mer 5'-ggt cga ggg acc tat acc g-3' mutant lox site
31 En2-SA 20 mer 5'-atg aca acc agg tcc cag gt-3' En2 gene splice accepter
32 34W-1 34 mer 5'-ctg ttt gct acc taa aga agg cct ttt ttc tgg t-3' 34 bp size marker
33 34W-2 34 mer 5'-acc aga aaa aag gcc ttc ttt agg tag caa aca g-3' 34 bp size marker
34 HBx-1 20 mer 5'-tct acc gtc ccc ttc ttc at-3' HBV HBx gene
35 HBx-2 21 mer 5'-caa ttt atg cct aca gcc tcc-3' HBV HBx gene
36 Ori-3 21 mer 5'-cac aga atc agg gga taa cgc-3' pUC origin
37 EL-9 20 mer 5'-cct aga cgt tgt aag aag gc-3' mouse E-selectin
38 EL-11 20 mer 5'-gat tgg aca ctc aat gga tc-3' mouse E-selectin
39 bsr-1 21 mer 5'-ctt ctc tgt cgc tac ttc tac-3' blasticidin S-resistance gene (bsr)
40 bsr-2 20 mer 5'-gca gaa atc gga gga aga ag-3' blasticidin S-resistance gene (bsr)
41 bsr-3 20 mer 5'-caa ctc cct aca cat acc ac-3' blasticidin S-resistance gene (bsr)
42 AC-1 19 mer 5'-tcg gct tct ggc gtg tga c-3' AC promoter
43 AG-1 20 mer 5'-ggt tgt tgt gct gtc tca tc-3' rabbit beta-globin partial genomic DNA (intron/polyA signal)
44 AG-2 20 mer 5'-ctg cta acc atg ttc atg cc-3' rabbit beta-globin partial genomic DNA (intron/polyA signal)
45 GFP-1 20 mer 5'-tac ggc aag ctg acc ctg aa-3' GFP gene
46 GFP-2 20 mer 5'-tgt gat cgc gct tct cgt tg-3' GFP gene
47 AAT-7 18 mer 5'-gtg tct aag gct gac tgg-3' human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257]
48 AAT-21 20 mer 5'-cct ggt ctg aat gtg tgt gc-3' human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257]
49 AAT-31 18 mer 5'-tgt ccc agg tca gtg gtg-3' human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257]
50 PAC-1 20 mer 5'-tcc atg acc gag tac aag cc-3' puromycin N-acetyltransferase (pac) gene
51 PAC-2 20 mer 5'-ggc ctt cca tct gtt gct gc-3' puromycin N-acetyltransferase (pac) gene
52 bsd-1 19 mer 5'-ggt ctc cca tgc ctt tgt c-3' blasticidin S-resistance gene (bsr)
53 bsd-2 19 mer 5'-cac ata acc aga ggg cag c-3' blasticidin S-resistance gene (bsr)
54 AG-3 18 mer 5'-gat cct gag aac ttc agg-3' rabbit beta-globin partial genomic DNA (intron/polyA signal)
55 AG-4 19 mer 5'-acc acc ttc tga tag gca g-3' rabbit beta-globin partial genomic DNA (intron/polyA signal)
56 AG-5 19 mer 5'-tag gca gcc tgc acc tga g-3' rabbit beta-globin partial genomic DNA (intron/polyA signal)
57 AG-6 20 mer 5'-cag aag tca gat gct caa gg-3' rabbit beta-globin partial genomic DNA (intron/polyA signal)
58 RGpA-1 20 mer 5'-tca tag ctg tcc ctc ttc tc-3' rabbit beta-globin partial genomic DNA (intron/polyA signal)
59 RGpA-2 21 mer 5'-ctg ctg tcc att cct tat tcc-3' rabbit beta-globin partial genomic DNA (intron/polyA signal)
60 AG3AS 19 mer 5'-cct gaa gtt ctc agg atc c-3' rabbit beta-globin partial genomic DNA (intron/polyA signal)
61 pSP72-1 19 mer 5'-ggg ctg gct tta cta tgc g-3' pSP72
62 pSP72-2 20mer 5'-ata gtt aag cca gcc ccg ac-3' pSP72
63 pSP72-3 20 mer 5'-ata agg gcg aca cgg aaa tg-3' pSP72
64 pSP72-4 20mer 5'-agt aag ttg gcc gca gtg tt-3' pSP72
65 pSP72-5 21mer 5'-tca cgt taa ggg att ttg gtc-3' pSP72
66 pSP72-6 20 mer 5'-tgt agg tat ctc agt tcg gt-3' pSP72
67 AAT-8 20 mer 5'-acc tgg gac agt cta gaa ct-3' human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257]
68 AAT-9 20 mer 5'-ttg ccc ctt ctg tca ttc ac-3' human alpha-1-antitrypsin gene(AAT) promoter [GenBank Accession:D38257]
69 pUpA 20mer 5'-tat tcc ttt gcc ctc gga cg-3' mouse PGK gene polyadenylation signal
70 pUpA-S 24mer 5'-gca gaa att gat gat cta tta aac-3' mouse PGK gene polyadenylation signal
71 SA-1 24 mer 5'-tcc tct ttg tta ggg ttc ttc ttc-3' mouse Engraled-2 (En2) gene splice acceptor
72 SA-2 19 mer 5'-gcc ctt tct cct cca tga c-3' mouse Engraled-2 (En2) gene splice acceptor
73 SA-3 20 mer 5'-gca aac gag gcc aga aag at-3' mouse Engraled-2 (En2) gene splice acceptor
74 SA-4 20mer 5'-tta cat agg aag ggg acg ga-3' EN2 gene splice acceptor ※not work!
75 SA-5 21mer 5'-ggt cac ttt atg ttc ttg ccc-3' mouse Engrailed-2 (En2) gene splice acceptor
76 SA-6 20mer 5'-agg tct caa gtt tta gcc gg-3' mouse Engrailed-2 (En2) gene splice acceptor
77 SA-7 20mer 5'-atg tcc tct ggt gct caa ag-3' mouse Engrailed-2 (En2) gene splice acceptor
78 SA-8 20 mer 5'-ata gga gtt tgc gga ggt tg-3' mouse Engrailed-2 (En2) gene splice acceptor
79 SA-9 28 mer 5'-ata gtc gac ttc ttc ttt ggt ttt cgg g-3' mouse Engrailed-2 (En2) gene splice acceptor
80 SA-10 19 mer 5'-agc agt gaa ggc tgt gcg a-3' mouse Engrailed-2 (En2) gene splice acceptor
81 SA-11 20 mer 5'-ccc gaa aac caa aga aga ag-3' mouse Engrailed-2 (En2) gene splice acceptor
82 LZUS-3 19 mer 5'-cgc atc gta acc gtg cat c-3' lacZ gene
83 STOP-1 29 mer 5'-taa ctg agt aga cta gtc tag aat tcg gc-3' universal terminator
84 STOP-2 33 mer 5'-tcg agc cga att cta gac tag tct act cag tta-3' universal terminator
85 polyT-Xho 24 mer 5'-ccc ctc gag ttt ttt ttt ttt ttt-3' polyT(15) + XhoI site
86 polyT20-Xho 29 mer 5'-ccc ctc gag ttt ttt ttt ttt ttt ttt tt-3' polyT(20) + XhoI site
87 VC-11 20mer 5'-caa cga tct ctg tac atc cc-3' vascular cell adhesion molecule (VCAM)
88 VC-12 20mer 5'-aga ggc tgt aca ctc tgc ct-3' vascular cell adhesion molecule (VCAM)
89 VC-13 21mer 5'-ccc ttg aac aga tca atc tcc-3' vascular cell adhesion molecule (VCAM)
90 VC-15 20mer 5'-gca gag act tga aat gcc tg-3' vascular cell adhesion molecule (VCAM)
91 Amp-S 20mer 5'-tac agg cat cgt ggt gtc ac-3' ampicillin resistance gene
92 Amp-AS 20mer 5'-aaa tgt gcg cgg aac ccc ta-3' ampicillin resistance gene
93 Primer3 24mer 5'-ccc gtg gtg gtg ttc gac ttt gcc-3' HSV
94 Primer4 24mer 5'-gca caa aga tgg agt ccg tgt ccc-3' HSV
95 154-1 23mer 5'-ttc ttc gtc aag gct cac gtg cg-3' HSV
96 154-2 25mer 5'-ccg agt tac aca cga cct tga tgg c-3' HSV
97 297-1 26mer 5'-aag gtc gtg tgt aac tcg gtg tac gg-3'HSV
98 297-2 23mer 5'-gat gat gcg cat gga ata ggg cc-3' HSV
99 hyg-pA 20mer 5'-tgc ctt cct tga ccc tgg aa-3' pIRES1hyg
100 FRT-S20mer5'-gct tca aaa gcg ctc tga ag-3' FRT
101AUAP20mer 5'-ggc cac gcg tcg act agt ac-3' abridged universal amplification primer for 5'-RACE
102Flp-2 20mer 5'-tcg gaa gaa gca gat aag gg-3' Flp gene
103Flp-5 20mer 5'-atc cta ccc ctt gcg cta aa-3' Flp gene
104Z-5 20mer 5'-acg gcg gat tga ccg taa tg-3' lacZ gene
105PGKpA22mer5'-aac ttccat ttt agt gga cat c-3' pU-21T pA2
106SA-1220mer5'-gga aac aaa ctt ggc ctc ac-3'mouse Engraled-2 (En2) gene splice acceptor
107SA-1320mer5'-cct gtt ggt ctg aaa ctc ag-3' mouse Engraled-2 (En2) gene splice acceptor
108Z-620mer5'-gcg att aag ttg ggt aac gc-3' lacZ gene
109GFP-320mer5'-acg aga agc gcg atc aca tg-3' GFP gene
110Z-319mer5'-ggc ctc ttc gct att acg c-3' lacZ gene
111Z-720mer5'-tct ggc ctt cct gta gcc ag-3' lacZ gene
112Z-821mer5'-gtt tta caa cgt cgt gac tgg-3' lacZ gene
113SV40pA-S21mer5'-gcc ata cca cat ttg tag agg-3' pU-21T pA1
114DT-A-120mer5'-tac cac ggg act aaa cct gg-3' DT-A gene
115DT-A-220mer5'-cgc tta acg ctt tcg cct gt-3' DT-A gene
116DT-A-321mer5'-gtt gat gga gca agt cgg aac-3' DT-A gene
117DT-A-421mer5'-cct tgt gta cca gat ttt ggc-3' DT-A gene
118DT-A-520mergaa aac gtg gcc aag atg cg-3' DT-A gene
119PGKproSizeSequenceNote
120PGKpA-S22mer5'-aag atg tcc act aaa atg gaa g-3' pU-21T pA2
121GFP-1AS20mer5'-ttc agg gtc agc ttg ccg ta-3' GFP gene
122SA14AS21mer5'-cac atc cat gct gag gat gag-3' mouse Engraled-2 (En2) gene splice acceptor
123SA-ter-Kp32mer5'-atg gta cct aag cag tga agg cta tgc gag gc-3'mouse Engraled-2 (En2) gene splice acceptor
124WZ-1421mer5'-gct ctc tac agg tgg atc aag-3' pU-21W
125WZ-1721mer5'-gca gtt caa tca gct gct ttc-3' pU-21W
126SA-12520merctc ctg gga act cta ctg cc-3' mouse Engraled-2 (En2) gene splice acceptor
127SA-125AS20merggc agt aga gtt ccc agg ag mouse Engraled-2 (En2) gene splice acceptor
128pSP72-4R20mer5'-aac act gcg gcc aac tta ct-3' pSP72
129LPBL27mer5'-gga tcc gga acc ctt aat ata act tcg-3' mouse Engraled-2 (En2) gene splice acceptor
130Sry-F20mer5'-tga ctg gga tgc agt agt tc-3' mouse Sry gene
131Sry-R20mer5'-tgt gct aga gag aaa ccc tg-3' mouse Sry gene
132pSP72-721mer5'-tca agg atc tta ccg ctg ttg-3' pSP72
133pSP72-821mer5'-gtg aaa acc tct gac aca tgc-3' pSP72
1343RACE-AP30mer5'-ggc cac gcg tcg act agt act ttt ttt ttt-3' 3'-RACE
135SA12-AS20mer5'-gtg agg cca agt ttg ttt cc-3' mouse Engraled-2 (En2) gene splice acceptor
136SA1421mer5'-ctc atc ctc agc atg gat gtg-3' mouse Engraled-2 (En2) gene splice acceptor
137SA8-AS20mer5'-caa cct ccg caa act cct at-3' mouse Engraled-2 (En2) gene splice acceptor
138iCre-120mer5'-agg cct tct ctg aac aca cc-3' iCre
139iCre-221mer5'-ttg gtc ctg cca atg tgg atc-3' iCre
140SA3AS20mer5'-atc ttt ctg gcc tcg ttt gc-3' mouse Engraled-2 (En2) gene splice acceptor
141SA3.5AS23mer5'-aag agt agg cta gcc agt caa gg-3' mouse Engraled-2 (En2) gene splice acceptor
142SA4.5AS25mer5'-ttc ctg tcc agg aga ggg acc tgg c-3' mouse Engraled-2 (En2) gene splice acceptor
143SA5AS21mer5'-ggg caa gaa cat aaa gtg acc-3' mouse Engraled-2 (En2) gene splice acceptor
144SA6AS22mer5'-ccg gct aaa act tga gac ctt c-3' mouse Engraled-2 (En2) gene splice acceptor
145SA7AS23mer5'-tct ttg agc acc aga gga cat cc-3' mouse Engraled-2 (En2) gene splice acceptor
No.NameSizeSequenceNote

[更新2014.10.27]