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研究発表を行った学会；
・2016 AMMRA & AMPC meeting in Hakone
　2016年5月20日〜21日（箱根）

タイトル；Efficient animal production using oocyte cryopreservation derived from ultra-superovulation technique in mice.
発表者；椋木　歩　氏
　　　（熊本大学　生命資源研究・支援センター　資源開発分野）
要旨；
Ultra-superovulation technique using inhibin antiserum (IAS) and equine chorionic gonadotropin (eCG) produced more than 100 oocytes from single female mice (Takeo and Nakagata, PLoS ONE, 2015). In this study, we examined viability, fertility and developmental ability of cryopreserved mouse oocytes derived from coadministration of IAS and eCG (IASe). IASe yielded about five times higher number of oocytes than eCG. N-acetyl-L-cysteine improved fertilization rate of the vitrified-warmed oocytes. Obtained two-cell embryos developed to blastocysts in vitro. In summary, oocyte cryopreservation derived from IASe will be applicable to efficient production of mice by in vitro fertilization and embryo transfer.