アクティブボード・２０１４年　５月
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研究発表を行った学会； The 78th Annual Scientific Meeting of the Japanese Circulation Society(JCS2014)
　２０１４年３月２１日〜２３日（東京）
タイトル；Periadventitial Adipose Tissue-Derived Angiopoietin-like protein 2 Enhances the Neointimal Formation after Endovascular Injury.
発表者；田 哲　氏
　　　（熊本大学　大学院生命科学研究部　分子遺伝学分野）
Abstract；
Background: Perivascular adipose is distributed ubiquitously around arteries throughout the body. Recently, it was reported that inflammatory change in the perivascular adipose has a crucial role in the pathogenesis of vascular disease. More recently, we reported that Angiopoietin-like protein2 (Angptl2), which is abundantly expressed in visceral adipose tissue, causes chronic adipose tissue inflammation and subsequent metabolic abnormalities in obesity.
Methods and Results: We examined that Angptl2 was abundantly expressed in pericardial adipose tissue. Next, we examined whether perivascular adipose tissue-derived Angptl2 affects neointimal hyperplasia formation after endovascular injury. To do so, firstly we performed wire injury using Angptl2-deficient mice (Angptl2-/-) and adipose tissue-specific over expressed Angptl2 transgenic mice (aP2-Angptl2). Angptl2-/- mice exhibited a light neointimal formation, however aP2-Angptl2 mice exhibited a serious neointimal formation. In order to get rid of the effect of femoral artery derived-Angptl2, we replaced the perivascular adipose tissue with exogeneous epidydimal adipose tissue of Angptl2-/- mice, those of aP2-Angptl2 mice, or those of their wildtype littermate mice. Compared to adipose tissue from wildtype mice, expression of proinflammatory factors in Angptl2-/- adipose tissue significantly decreased, and Angptl2-/- adipose tissue attenuated neointimal hyperplasia after endovascular injury. Conversely, aP2-Angptl2 significantly increased expression of proinflammatory factors, and enhanced neointimal hyperplasia after endovascular injury. And both transplanted adipose tissue regulated vascular MMP2 expression and activity.
Conclusions: Our findings suggest that perivascular adipose tissue derived-Angptl2 might contribute to vascular inflammation, leading to neointimal hyperplasia by regulating MMP2 after angioplasty.