アクティブボード・２０１４年　３月
　　　　　・・・・・２０１４年　３月　２日更新・・・・・

研究発表を行った学会；
・第8回トランスポーター研究会年会
　２０１３年　６月１５日〜１６日（熊本）
タイトル；A specific amino acid metabolic state of human ES/iPS cells and its significance.
発表者；白木　伸明　氏
　　　（熊本大学　発生医学研究所　多能性幹細胞分野）
Abstract；
Human embryonic stem (hES) cells and induced pluripotent stem (iPS) cells are invaluable resources in regenerative medicine; however, their utility is hindered by marked differences in differentiation propensities between cell lines. Here, we identified a specific dependence of the pluripotent hES/iPS cells on a particular amino acid for their growth. We have established a differentiation procedure for hES/iPS cells to differentiate into the definitive endoderm derivatives of the pancreas and liver. To establish a more endoderm selective differentiation method, we focused on the differences of amino acid metabolisms between the undifferentiated and differentiated definitive endoderm cells. When cultured in media deprived of the particular amino acid, undifferentiated hES/iPS cells rapidly ceased to proliferate and apoptosis occurred, which resulted in an increased proportion of Sox17-positive definitive endoderm cells. Moreover, the elimination of undifferentiated cells using the particular amino acid deprived media was applicable to several different hES/iPS cell lines.
In this study, a novel hES/iPS cell differentiation system, based on the difference of a specific amino acid metabolism between undifferentiated cells and differentiated cells, is established. By using the media deprived of a particular amino acid, undifferentiated cells can be specifically eliminated during endoderm differentiation. This method is useful for directing human ES/iPS cell lines that are resistant to differentiation to achieve a higher differentiation efficiency.