アクティブボード・２０１３年１０月
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研究発表を行った学会；
・第４６回日本発生生物学会年会
　２０１３年　５月２８日〜３１日（松江）

タイトル；Investigation of role of a novel pancreatic beta cell gene.
発表者；大森　久嘉　氏
　　　（熊本大学　発生医学研究所　多能性幹細胞分野）
Abstract；
We developed an efficient in vitro procedure for directed differentiation of ES cells into the definitive endoderm (DE) and then Pdx1-expressing pancreatic progenitor cells using M15 cells. Mouse ES cell-derived DE and Pdx1-expressing cells were isolated and their gene expression profiles were studied by DNA microarray analysis. Genes that were specifically expressed in the DE and/or in Pdx1-expressing cells were extracted. We checked their expression patterns during normal embryonic development by in situ hybridizations. As a result, we identified several novel genes that expressed in the E8.5 mouse embryos and in distinct domains in the pancreas at E14.5 embryos (Ogaki et al., 2011). However, the functions of these genes are unclear. Here, we examined the function of these novel genes.
We would like to report a gene, c2cd4b, which is expressed in Insulin-expressing cells. C2cd4 family consist of 4 subfamily members, c2cd4a, c2cd4b, c2cd4c and c2cd4d. We found that c2cd4a and c2cd4c are also expressed in the mouse pancreatic islets.
To investigate the function of c2cd4b, we examined the intracellular localization of c2cd4 gene family. Overexpression of a HA-tagged c2cd4a and c2cd4b revealed their nuclear localization. By contrast, c2cd4c was localized in the cytoplasm.
To check the function of c2cd4 on pancreatic differentiation, we are trying to establish a Tet-inducible c2cd4 ES cell line and would like to discuss our results.