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研究発表を行った学会；
・Single Cell Analysis Summit
　２０１２年　９月２５日〜２６日（San Diego, USA）

タイトル；Characterization of gene expression profiles for different types of mast cells pooled from mouse stomach subregions by an RNA amplification method.
発表者；土屋　創健　氏
　　　（熊本大学　大学院生命科学研究部　薬学生化学分野）
Abstract；
Mast cells (MCs) play pivotal roles in allergy and innate immunity and consist of two heterogenous subclasses, connective tissue type MCs (CTMCs) and mucosal MCs (MMCs). However, the molecular basis determining the different characteristics of these multiple MC subclasses remains unclear. To approach this, we developed a method of RNA extraction/amplification for intact in vivo MCs pooled from frozen tissue sections, which enabled us to obtain the global gene expression pattern of pooled MCs belonging to the same subclass. MCs were isolated from the submucosa (CTMCs) and mucosa (MMCs) of mouse stomach sections, respectively, 15 cells were pooled, and their RNA was extracted, amplified and subjected to microarray analysis. Known marker genes specific for MMCs and CTMCs showed expected expression trends, indicating accuracy of the analysis. We identified 1,272 genes showing significantly different expression levels between CTMCs and MMCs, and classified them into clusters on the basis of similarity of their expression profiles compared with bone marrow-derived MCs (BMMC), which are the cultured MCs with so-called ‘immature’ properties. Among them, we found that several key genes such as Notch4 had CTMC-biased expression and Ptgr1 had MMC-biased expression. Furthermore, there is a difference in the expression of several genes including extracellular matrix protein components, adhesion molecules, and cytoskeletal proteins between the two MC subclasses, which may reflect functional adaptation of each MC to the mucosal or submucosal environment in the stomach.