アクティブボード・２０１２年　５月
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研究発表を行った学会；
・KEY Forum in Developmental Biology and Regenerative Medicine
　２０１１年　９月　８日〜９日（熊本）

タイトル；Generation of insulin-producing cells from iPS and ES cells by protein transduction method.

発表者；貝塚　拓　氏
　　　（熊本大学　大学院生命科学研究部　分子生理学分野）
Abstract；
It is desired that induced-pluripotent stem (iPS) cells are applied to the regenerative medicine for treatment of patients with diabetes. However, the useful and safe differentiation method has not been fully developed. In this study, we tried to establish the effective differentiation method by protein transduction of three transcriptional factors (Pdx-1, NeuroD, MafA), which are important for the development of pancreatic β-cell. This method has no risk for unexpected genetic modifications by the exogenous DNA in the target cells. Protein transduction domain fused proteins were efficiently transferred into embryonic stem (ES) cells and iPS cells. Protein transduction of the three factors induced the differentiation of mouse ES and mouse/human iPS cells to insulin-producing cells. Furthermore, laminin-5-rich extracellular matrix efficiently induced the differentiation under feeder-free conditions. The cell differentiation was confirmed with the expressions of both protein and mRNA of insulin in addition to marker genes in pancreatic-β cells such as Pdx-1, MafA, Glut2, Kir6.2, SUR1 and IAPP. Present results suggest that direct delivery of recombinant proteins is useful for the differentiation of ES and iPS cells into insulin-producing cells.