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研究発表を行った学会；
・第７６回日本循環器学会学術集会
　２０１２年　３月１６日〜１８日（福岡）

タイトル；Periadventitial Adipose Tissue-Derived Angiopoietin-like protein 2 Enhances
the Neointimal Formation after Endovascular Injury.

発表者；田　哲　氏
　　　（熊本大学　大学院生命科学研究部　分子遺伝学分野、心臓血管外科学分野）
Abstract；
Background: Periadventitial adipose is distributed ubiquitously around arteries throughout the body. Recently, it was reported that inflammatory change in the periadventitial adipose has a crucial role in the pathogenesis of vascular disease. More recently, we reported that Angiopoietin-like protein2 (Angptl2), which is abundantly expressed in visceral adipose tissue, causes chronic adipose tissue inflammation and subsequent metabolic abnormalities in obesity.
Methods and Results: We examined whether Angptl2 is expressed in human periadventitial/pericardial adipose tissue by immunohistochemical analysis. Expectedly, we found that Angptl2 was abundantly expressed in pericardial adipose tissue. Next, we examined whether periadventitial adipose tissue-derived Angptl2 affects neointimal hyperplasia formation after endovascular injury. To do so, we replaced the periadventitial adipose tissue with exogeneous epidydimal adipose tissue of Angptl2-deficient mice, those of adipose tissue-specific overexpressed Angptl2 transgenic mice, or those of their wildtype littermate mice fed on normal chow diet. Compared to adipose tissue from wildtype mice, expression of proinflammatory factors in Angptl2-deficient adipose tissue significantly decreased, and attenuated neointimal hyperplasia after endovascular injury. Conversely, adipose tissue-overexpressing Angptl2 significantly increased expression of proinflammatory factors, and enhanced neointimal hyperplasia after endovascular injury.
Conclusions: Our findings suggest that periadventitial adipose tissue derived-Angptl2 might contribute to vascular inflammation, leading to neointimal hyperplasia after angioplasty.