アクティブボード・２０１１年　９月
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研究発表を行った学会；
・第１４回国際免疫学会議
　２０１０年　８月２２日〜２７日（神戸）

タイトル；Generation of dendritic cells and macrophages from human iPS cells aiming at application to cell therapy.

発表者；千住　覚　氏
　　　（熊本大学　大学院生命科学研究部　免疫識別学分野）
Abstract；
We previously established methods to generate functional dendritic cells (DC) from mouse, monkey, and human embryonic stem (ES) cells. We designated them as ES-DC and mouse models have demonstrated the induction of anti-cancer immunity and prevention of autoimmune disease by in vivo administration of genetically engineered ES-DC. The histoincompatibility between patients to be treated and available human ES cells and also the ethical concerns related to the use of human ES cells are anticipated to be serious obstacles in the clinical application of ES-DC. However, we will be able to resolve these issues by applying recently developed iPS cell technology. In the current study, we generated human iPS celles by lentivirus-mediated introduction of genes for Oct3/4, Sox2, Klf4, and c-Myc into dermal fibroblasts. The established iPS cell clones were subjected to differentiation culture that we previously developed for human ES cells. As a result, we generated iPS cell-derived DC (iPS-DC) with characteristics of DC including capacity of T cell-stimulation, cytokine production, and antigen-presentation. A xeno-free procedure to generate human iPS-DC was also established, which will be required in the future application of this technology to clinical immune-therapy. In addition, we generated iPS cell-derived macrophages (iPS-MP) genetically modified to possess specific functions useful for therapy of cancer and Alzheimer disease.