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研究発表を行った学会；
・第４回神経発生討論会
　２０１０年　３月１９日〜２０日（岡崎）

タイトル；Novel membrane protein Gsg1 is essential for neuronal migration and dendrite formation of cortical pyramidal neurons.
　
発表者；　渡辺　啓介　氏
　　　（熊本大学　大学院生命科学研究部　脳回路構造学分野）

Abstract；
Mammalian neocortex consists of two types of neurons: glutamatergic and GABAergic neurons. During corticogenesis, cell type-specific regulation of stepwise processes such as neural migration and dendrite formation is crucial for functional organization of the neocortex. In order to elucidate specific molecular mechanism underlying development of cortical glutamatergic neurons, we tried to find genes expressed specifically in developing glutamatergic neural lineages by single cell microarray analysis. Among these genes, we identified transmembrane protein glutamatergic neuron-specific gene (Gsg1), the C. elegans homolog, which regulates initial neuroblast polarity in C. elegans. However, involvement of Gsg1 in development of the mammalian neocortex remains unknown. Gsg1 is strongly expressed in glutamatergic neural lineage cells in the embryonic neocortex. Downregulation of Gsg1 mediated by shRNA results in defects of radial migration and dendrite formation of glutamatergic neurons in vivo. Whereas Gsg1 protein is localized also in the cortical axons, we cannot find remarkable abnormal axonal trajectory of callosal neurons in this study. These results suggest that Gsg1 is required for proper radial migration and dendrite formation of glutamatergic neurons during development.