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研究発表を行った学会；
・第３２回日本分子生物学会年会
　２００９年１２月９日〜１２日（横浜）

タイトル；Screening of compounds that affect the localization of poly(A)+RNA in the nuclear speckles.
　
発表者；　三原　由揮　氏
　　　（熊本大学　大学院自然科学研究科　ＲＮＡ分子生物学研究室）

Abstract；
　Nuclear speckles are non-membrane structures present in the nuclei of eukaryotic cells. Nuclear speckles contain poly(A)+RNA (mRNA and non-coding RNA) and various RNA processing factors. It is thought that nuclear speckles play important roles in gene expression.
To analyze the functions and the formation mechanisms of nuclear speckle, we screened 3,500 samples of the Actinomycetes culture supernatants that affect the localization of poly(A)+RNA. We also screened 1,000 supernatant samples that affect the localization of SC35.
Of 3,500 samples screened, we identified 64 samples that affect the localization of poly(A)+RNA in the nuclear speckles. Those samples caused accumulation of poly(A)+RNA in the enlarged speckles, dispersion of poly(A)+RNA from the speckles or aggregation of poly(A)+RNA in the cytoplasm.
In the cells treated with the supernatant 1900-17a, poly(A)+RNA was dispersed from the nuclear speckles. Nuclear distributions of SC35 and SF2, splicing factors localized in the nuclear speckles, were also changed to become rounded and smaller. Based on the changing of the localization of splicing factors and poly(A)+RNA, we investigated pre-mRNA splicing patterns of some genes using RT-PCR. Alternative splicing patterns of several genes analyzed, however, were not significantly changed.
Purification of the active compounds from these supernatants and identification of the target factors for the compounds are now underway.