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研究発表を行った学会；
・41st Annual Meeting for the Japanese Society of Developmental Biologists.
　2008年5月28日~30日(徳島)

タイトル；Guided differentiation of ES cells into Ngn3-rxpressing pancreatic precursors.
　
発表者；　樋口　裕一郎　氏
　　　（熊本大学　発生医学研究センター　幹細胞制御分野）
Abstract；
　Embryonic stem (ES) cells have a virtually unlimited potential to proliferate as well as to produce most differentiated cell types in our body, including the pancreatic cell lineage. Recently, others reported for the in vitro generation of cells expressing insulin, a pancreatic β cell marker from human ES cells (D'Amour et al., 2006), but the molecular mechanisms for each inductive process remained much unknown.
We had established an efficient in vitro differentiation procedure to induce Pancreatic and duodenal homeobox gene 1 (Pdx1)-expressing pancreatic progenitor cells from embryonic stem (ES) cells, by using a co-culture system with a mesodermal cell line and the addition of growth factors (Shiraki et al., 2008). However, it was not shown how to efficiently induce the Pdx1-expressing cells to differentiate in vitro into pancreatic β cells.
Here, we established an efficient in vitro differentiation procedure to induce Ngn3-expressing pancreatic endocrine progenitor cells from ES cells, by using a co-culture system and serum-free medium condition. To check whether the Ngn3 -expressing cells were derived from endoderm cell lineage, ES cell-derived E-cadherin+/PDGFRα+ mesendodermal cells or E-cadherin+/CXCR4+ definitive endoderm cells were recovered and cultured under serum-free condition. Then, we found that both mesendoderm and definitive endoderm fractions gave rise to Ngn3-expressing cells, thus confirmed that the ES cell-derived Ngn3-expressing cells generated in the present procedure were of endodermal origin.