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研究発表を行った学会；
・21st International Mammalian Genome Conference、2007年10月28日~11月1日(京都)

タイトル；EGTC, Database for the Exchangeable Gene Trap Clones; Resource of mouse and ES cell lines for the functional analysis of the mouse genome.

発表者；　荒木　正健　氏
　　　（熊本大学　生命資源研究・支援センター　バイオ情報分野）
Abstract；
　Gene trapping in ES cells is a proven method for large-scale random insertional mutagenesis in the mouse genome. Gene trap vectors contain a promoter-less reporter gene downstream of a splice acceptor and a selectable marker gene. A fusion transcript between the integrated (trapped) gene and the reporter gene can be easily cloned by 5’-RACE. We have developed exchangeable gene trap vectors, pU18, pU21, pU21B, pU21T and pU22. The reporter β-geo gene can be exchanged into any other DNA of interest through Cre-mediated recombination. We have isolated trap ES clones, determined sequence tags (GSSs) of trapped genes by 5’-RACE, and annotated them by BLAST and BLAT search. The data of trap clones are opened as a Database for the Exchangeable Gene Trap Clones, EGTC (http://egtc.jp). Until the end of July 2007, total 310 GSSs have been registered. Among them, 73% are known genes, 19% are ESTs, and 8% are new genes. 249 GSSs were determined their chromosomal localization through BLAT search. Since the pU21, pU21B and pU21T vectors have stop codons in upstream of the start codon of the β-geo, the integration sites of the vectors showed a strong bias to the vicinity of the ATG exon of trapped genes, indicating that these vectors could induce a null allele efficiently. In addition to determination of GSSs, more than 130 trap mouse lines have been established and deposited to the CARD R-BASE; the database for cryopreserved embryos.