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研究発表を行った学会； 第３６回日本免疫学会総会・学術集会
　　　２００６年１２月１１日〜１３日（大阪）
タイトル； Role of G5PR in the survival of T cells in the early T lineage cell development.
発表者；　WANG Xiaodan　氏
　　　（熊本大学　大学院医学薬学研究部　免疫学分野）
Abstract；
G5PR is a protein phosphatase component associated with the catalytic fragment of PP2A and PP5, and is involved in protection of activation induced cell death of B-cells after BCR crosslinkage. Lack of G5PR caused prolonged activation of JNK and Bim induced by BCR crosslinkage and resulted in increased apoptotic cell death. Here, we prepared the mice of conditionally targeted the g5pr gene in T lineage cells by combination with Lck-cre transgenic mice. The mutant mice displayed the dramatic decrease of thymocytes in the double positive (DP) and single positive (SP) populations. To study whether this decrease is caused by the impairment of T lineage cell survival or increased of cell apoptosis during the positive selection or negative selection processes, we made the mutant mice with transgenic introduction of the rearranged and functional TCRαβ genes of anti-HY specificity (G5PR-T-KO/TCRαβ mice) and characterized the rescue of DP cell population, suggesting the G5PR is necessary for the survival of T cells during DP to SP transition presumably in the positive selection process. This mutant mouse would provide a model to study the molecular mechanism regulating the selection and maintenance of central tolerance.